Open Access Research

Multiphasic analysis of the temporal development of the distal gut microbiota in patients following ileal pouch anal anastomosis

Vincent B Young12*, Laura H Raffals3, Susan M Huse4, Marius Vital5, Dongjuan Dai6, Patrick D Schloss2, Jennifer M Brulc7, Dionysios A Antonopoulos7, Rose L Arrieta8, John H Kwon8, K Gautham Reddy8, Nathaniel A Hubert8, Sharon L Grim4, Joseph H Vineis4, Sushila Dalal8, Hilary G Morrison4, A Murat Eren4, Folker Meyer7, Thomas M Schmidt6, James M Tiedje5, Eugene B Chang8 and Mitchell L Sogin4

Author Affiliations

1 Department of Internal Medicine, Division of Infectious Diseases, Ann Arbor, MI, USA

2 Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, MI, USA

3 Department of Internal Medicine, Division of Gastroenterology and Hepatology, Mayo Clinic, Rochester, MN, USA

4 Josephine Bay Paul Center, Marine Biological Laboratory, Woods Hole, MA, USA

5 Center for Microbial Ecology, Michigan State University, East Lansing, MI, USA

6 Department of Microbiology and Molecular Genetics, Michigan State University, East Lansing, MI, USA

7 Institute for Genomics and Systems Biology, Argonne National Laboratory, Argonne, IL, USA

8 Department of Medicine, Section of Gastroenterology, The University of Chicago, Knapp Center for Biomedical Discovery, Chicago, IL, USA

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Microbiome 2013, 1:9  doi:10.1186/2049-2618-1-9

Published: 4 March 2013

Abstract

Background

The indigenous gut microbiota are thought to play a crucial role in the development and maintenance of the abnormal inflammatory responses that are the hallmark of inflammatory bowel disease. Direct tests of the role of the gut microbiome in these disorders are typically limited by the fact that sampling of the microbiota generally occurs once disease has become manifest. This limitation could potentially be circumvented by studying patients who undergo total proctocolectomy with ileal pouch anal anastomosis (IPAA) for the definitive treatment of ulcerative colitis. A subset of patients who undergo IPAA develops an inflammatory condition known as pouchitis, which is thought to mirror the pathogenesis of ulcerative colitis. Following the development of the microbiome of the pouch would allow characterization of the microbial community that predates the development of overt disease.

Results

We monitored the development of the pouch microbiota in four patients who underwent IPAA. Mucosal and luminal samples were obtained prior to takedown of the diverting ileostomy and compared to samples obtained 2, 4 and 8 weeks after intestinal continuity had been restored. Through the combined analysis of 16S rRNA-encoding gene amplicons, targeted 16S amplification and microbial cultivation, we observed major changes in structure and function of the pouch microbiota following ileostomy. There is a relative increase in anaerobic microorganisms with the capacity for fermentation of complex carbohydrates, which corresponds to the physical stasis of intestinal contents in the ileal pouch. Compared to the microbiome structure encountered in the colonic mucosa of healthy individuals, the pouch microbial community in three of the four individuals was quite distinct. In the fourth patient, a community that was much like that seen in a healthy colon was established, and this patient also had the most benign clinical course of the four patients, without the development of pouchitis 2 years after IPAA.

Conclusions

The microbiota that inhabit the ileal-anal pouch of patients who undergo IPAA for treatment of ulcerative colitis demonstrate significant structural and functional changes related to the restoration of fecal flow. Our preliminary results suggest once the pouch has assumed the physiologic role previously played by the intact colon, the precise structure and function of the pouch microbiome, relative to a normal colonic microbiota, will determine if there is establishment of a stable, healthy mucosal environment or the reinitiation of the pathogenic cascade that results in intestinal inflammation.

Keywords:
Pouchitis; Microbiome; Microbial ecology; Inflammatory bowel disease